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Lower levels of IGF-1 and growth hormone in older adults have been linked with (5, 14): increased fat mass increased cardiovascular risk decreased muscle mass and strength lower cognitive function lower bone density limited joint mobility Several of these traits are seen in frail older adults

doi: 10.2174/1381612033454775 26 BaggioLLHuangQBrownTJDruckerDJBaggioLLHuangQet al

To interrogate the relative importance of consensus structure interactions for receptor binding and activation, we employed receptor mutagenesis, where each residue within the TMD that formed an interaction with any of the four peptides in the static cryo-EM structures was mutated to alanine (with the exception of A368 6.57 , which was mutated to glycine), and the binding affinity and cAMP signalling of each peptide was assessed