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In brief, heart tissue was homogenized in ice-cold 6% perchloric acid, centrifuged at 1000 rpm for 15 min at 0.5 C, and the supernatant fluid was injected into HPLC after neutralization to pH 67
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95% of the control group of rats experienced severe or medium-severe cerebral ischemia following occlusion of the carotid arteries
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