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Throughout these disease processes, ROS-mediated impairment of antioxidant defense systems and pro-survival pathways further amplifies the neurodegeneration cascade [339]
Fixation and permeabilization (15 min fixation, RT) were carried out with the Foxp3/Transcription Factor Staining Buffer set (eBioscience catalogue number 00-5523-00), followed by intracellular staining in permeabilization buffer for 30 min at 4 C
After the injection, instead of just putting the cap back on the needle, take a moment to gently massage the area for a minute
Ultrasound assessment of gastric content and volume
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