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In order to compare the three different assays, we used the established rat model of angiotensin-II-induced hypertension that was previously fully characterized regarding the increased blood pressure, impaired vascular function and aggravated inflammation as well as oxidative stress, including uncoupling of endothelial nitric oxide synthase, crosstalk of phagocytic NADPH oxidase with mitochondrial ROS formation [Citation2224], and the central role of myelomonocytic cells [Citation25]

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Measurable reductions in visceral fat on imaging typically become apparent by week 16 to 26