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A 4 l of sample was loaded onto a glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids using a FEI Vitrobot with an incubation time of 12 sec at 4 C and 100 % humidity

In addition, the activation of pancreatic stellate cells (PSCs) is a key mechanism for pancreatic islet fibrosis