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ROS assays As directed by the manufacturer, a Reactive Oxygen Species (ROS) assay kit (Beyotime, S0033S, Shanghai, China) was used to measure intracellular ROS levels based on the formation of the fluorescent compound dichlorofluorescein (DCF) under the peroxide-dependent oxidation of DCFH-DA
It is extensively studied in preclinical models for its role in cellular protection, signaling pathways, and tissue-specific stress response research
They come primarily from industry-sponsored cosmetic studies rather than large, independent, placebo-controlled dermatological trials
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