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W., Costill, D
This is based on its 6 to 8 day half-life and the pharmacological principle that 4 to 5 half-lives are required for near-complete elimination
The precipitate was collected (6000 rpm, 30 min, 4 C) and washed with 1 mL of acetone three times to remove the TCA
The consistency of your dosing depends on the accuracy of your initial mixing process
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