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Then, the cell viability was determined, and 100 L supernatant from cell culture medium was collected to measure LDH content using the specific kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturers protocol [28]
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Comparing the glutathione-scaffold site among a variety of other members of the thioredoxin superfamily also provides an explanation for their disulfide substrate preferences, for example, why glutaredoxins prefer GSSR in contrast to thioredoxins lacking most of the relevant residues (Fig
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