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The study demonstrated that KARs can form di-heteromers with both balanced (2:2) and unbalanced (3:1 and 1:3) stoichiometries and further proposed the potential existence of tri- and tetra-heteromeric assemblies

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The cofactors, either the coactivator SRC1 or the co-repressor NCoR, were expressed as fusions to the DNA binding domain of the yeast transcription factor GAL4

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