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Notably, upregulated genes in E9.25 Trim37 ko3 PGCs were enriched in pattern specification and mesenchyme development (Supplementary information, Fig

After blocking, membranes were incubated with primary and fluorescent-conjugated secondary antibodies for 1 h at room temperature with four 10-min washes with fresh 0.1% PBS-Triton X-100 after both the primary and secondary antibody incubations

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