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Solvent A was prepared by mixing 99 mL of Milli-Q (Millipore) water and 1 mL of 1 mol/L ammonium bicarbonate and was adjusted to a pH of 9.2 with 25% ammonium hydroxide (~0.1 mL)
A 1 L aliquot of supernatant extracted from the mitochondria was injected into the column, with the temperature maintained at 35 C and the flow rate set at 0.4 mL/min
It should be mentioned here that the body is able to produce glutathione itself, but as we explain below, this synthesis can be impaired by a number of factors, causing levels to fall
Ngi ang mun ci thin tng da, mun dng trng da bn vng, an ton, khng dng kem
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