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Further analysis by Western blot showed that rhGPx3 mutant with excess amino acid could be recognized by the monoclonal antibody against the hexahistidine tag, but not in the case of the protein treated with factor Xa (Figure 1a and 1b)
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Metabolite labeling was quantified by targeted LCMS analysis, with FAO activity calculated as the ratio of citrate ( m + 2) or malate ( m + 2) to palmitate ( m + 16), as described previously 53
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