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You should only increase your dose (titrate) when: You have successfully tolerated the current dose for at least 1-2 weeks

Each frozen tissue was weighed and dissolved in 200 L of lysing tissue solution (40 mg of EDTA in 1 mL of HClO4 and brought the volume to 100 mL using Milli-Q water) and then bead grinded for 2 min

ButeauJ , El-Assaad W, Rhodes CJ, Rosenberg L, Joly E, Prentki M

Compared to healthy controls, SCD patients have significantly lower erythrocyte GSH and cysteine concentrations 12 and a faster rate of GSH synthesis, suggesting that the endogenous cysteine supply is insufficient to meet all anabolic demands