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Incubate the plate at 37oC for 5 minutes
The lid domain and glycine-rich loop are critical for ATP binding, which triggers major shifts in their conformations (Galant et al., 2009)
To optimize the parameters of reduction reaction we studied the range of TCEP molar excess from 50 to 625 (Fig
These cells exhibit self-organizing, self-renewing and proliferative properties when cultured in an extracellular matrix (ECM) scaffold (typically Matrigel) in the presence of a specified cocktail of growth factors, in particular encompassing WNT-activating signals
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