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However, analyzing a dsbB mutant strain regarding its oxidation state and re-oxidation capacity in presence or absence of GSH or GSSG revealed that GSSG was still able to complement for the loss of DsbB, indicating a DsbB-independent mechanism (Supplementary figure 4)

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A QIAGEN midiprep was used to purify these plasmids at a high concentration, so a total of 3 ug DNA could be used per reaction

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