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Glutathione also helps regenerate other dietary antioxidants such as vitamins C and E.* This powerful antioxidant is essential for cells and tissues that experience high amounts of wear and tear on a daily basis, such as liver cells, cardiovascular tissues, and immune system cells

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

Moreover, stress can compromise the skins natural barrier function, making it more susceptible to environmental damage and dehydration

Additionally, some vitamins can interfere with the efficacy of other medications and even exacerbate side effects