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PCs ( P11) were seeded into collagen type IV and fibronectin coated dishes in Dulbeccos modified Eagles medium (DMEM, Life Technologies, Thermo Fisher Scientific, USA) supplemented with 20% FBS, 1% Glutamax (Life Technologies) and gentamicin (50 g/ml)

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Furthermore, administration of L-carnitine substantially altered the effects of TRZ and markedly reduced the expression of autophagy-related genes such as Beclin1, Autophagy Related Gene 5 (ATG5), and the inflammatory mediator TNF- in comparison to the TRZ treated group (Khedr et al