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If vitamin B 12 intake ceases, deficiency would usually not develop for at least 1-2 years, sometimes even longer

For the broader regulatory framework affecting peptide research in Canada and the United States, see FDA Peptide Reclassification 2026: What It Means for Canadian Researchers

[9] Visual disturbances have been reported in patients with Leber hereditary optic neuropathy, and rapid B12 administration may precipitate optic nerve atrophy in this genetically predisposed population, mandating extreme caution or alternative therapy

Research protocols typically use 250 to 500 mcg administered subcutaneously once or twice daily for 4 to 8 weeks