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On the day of the assay, cells were lifted, washed with 1X PBS, and stained (1:1000) with eBioscience Fixable Viability Dye eFluor 780 for 30 minutes on ice
Some studies analyzed the role of gut microbiota in acute kidney injury (AKI) (76) and also summarized the possible pathogenic pathways and mechanisms involved in the transition from AKI to CKD due to gut microbiota dysregulation (77)
Future work will be needed to validate these findings and to further explore the relationship of shared biomarkers between blood and brain
It includes Collagen 1&2, as well as Vitamins C, E, and A
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