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Luminescence was recorded for 45 min at 1 min intervals using a Fluoroskan Ascent FL (Thermo Scientific, Rockford, IL, USA) fluorimeter (excitation: 538 nm, emission: 604 nm), and the slopes were used for calculations

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Among these, cholesterol metabolism plays a context-dependent role in ferroptosis, with cholesterol and its intermediates either promoting or suppressing ferroptotic cell death depending on cellular and microenvironmental conditions