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In addition, to determine the percentage distribution of reacted RT between GSH and protein thiols, we treated HeLa cells with RT and lysed the cells using trichloroacetic acid to separate proteins from small molecules (Fig

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CD4 + CD25 - and CD4 + CD25 + T cells for transfer were obtained from splenocyte suspensions of SPF Wild type mice using a magnetic bead-based CD4 + T cell-isolation kit or the CD4 + CD25 + -Regulatory T cell-isolation Kit (Miltenyi Biotec) according to the manufacturers instructions