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Six weeks of a carefully planned cycle with degraded peptides produces uncertain results
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In Silico molecular Docking (MD) The binding affinity of n-hexadecanoic acid (the largest fatty acid contained in SP) and L-carnitine towards SIRT1 was modeled using Autodock Vina 1.1.2 39
Data availability All data analyzed in this publication were publicly available, including the human lung MSI and spotted mouse liver from METASPACE ( and the murine urinary bladder from the Proteomics Identifications Database (PXD001283) and the 10X Visium and MALDI (FMP-10 and DHB matrices) Parkinson mouse brain data ( The IDH -mutant tumor data are available from the BioImage Archive ( All data objects used for vignettes can also be found on the SpaMTP Zenodo community page ( Databases used for metabolite annotation include HMDB ( released November 2, 2021), ChEBI ( released September 1, 2023), LIPIDMAPS ( released September 15, 2023) and GNPS ( released April 21, 2025)
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