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Measurement of reactive oxygen species (ROS) and reduced glutathione (GSH) After maturation, the oocytes were incubated in 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) and 10 M Cell Tracker Blue (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 37 C in the dark to detect intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), respectively