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After culture for 24 h at 37 C and 5% CO 2 , cells on the upper surface of the membrane were carefully wiped off, whereas those on the undersurface of the membrane were fixed with 4% paraformaldehyde for 15 min, stained with 2% crystal violet for 5 min, and then rinsed under running water

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Supplementation with l-carnitine downregulates genes of the ubiquitin proteasome system in the skeletal muscle and liver of piglets