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Based on the manufacturers instructions (GenePharma, Shanghai, China), lentiviral (Lenti- RRM2 shRNA or Lenti-shRNA) (MOI = 15) was applied to transfect TPC-1 and IHH4 cells with 5 g/ml polybrene(Sigma, Cat# H9268) for 12 h
Some have disappeared altogether
Some patients space out doses (every 1014 days) to cut costs
NAC serves as a precursor for glutathione (GSH) synthesis, a critical antioxidant that neutralizes ROS and protects cells from lipid peroxidation
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