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These findings establish the mechanistic basis for the compound and provide proof of concept for its metabolic effects

This initial observation was further confirmed through side-by-side fluorescence scans of recombinant roGFP2 and roGFP2-iL in buffers containing either 10 mM DTT for full reduction or 10 mM H 2 O 2 for full oxidation of the probes, respectively (Figure 2)

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In multiple pathological settings, affected cells are not distributed randomly, but instead form contiguous zones of degeneration, synchronized tubular epithelial loss, aligned myofiber injury, or expanding fronts of tissue damage, patterns that are difficult to reconcile with strictly cell-autonomous execution