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cDNA was amplified using TaqMan Universal Master Mix II (Thermo Fisher Scientific) and ABI7900 (Life Technologies, Tokyo, Japan) according to the manufacturers instructions
Investigators attributed these negative results to peroxidative stress induced by the high ascorbate doses [147]
(13)(14) Nevertheless, GHRP-2 appears to induce desensitization at these receptors immediately after exposure, potentially reducing sensitivity lasting a complete four hours before reversal
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