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Bacteriostatic water maintains preservative efficacy for up to 28 days after first puncture when stored at 2-8C
According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 L of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 L of propidium iodide staining solution, which was also gently mixed
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