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For purification, a 5 ml GSTrap FF (GE Healthcare) was equilibrated with at least five column volumes of reducing lysis buffer

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Studies in animal models with gut microbiota depletion have shown increased plasma tryptophan, elevated brain serotonin concentrations, and reduced kynurenine pathway activity, all of which normalize following microbiota restoration (229231)

Exactly how much lighter depends on your starting weight, but people with obesity can expect about 3% body weight reduction, on average, by this point

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