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The column was pre-equilibrated with 20 mM HEPES, pH 7.4, 100 mM NaCl, 5 M tirzepatide, 100 M TCEP, 2 mM MgCl 2 , 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN, 0.00015% (w/v) CHS and 0.00025% digitonin

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