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Pierce Glutathion-Agarose reinigt effektiv hohe Mengen von berexprimierten GST-markierten Fusionsproteinen aus bakteriellen Lysaten, wie sie beispielsweise mit Thermo Scientific B-PER Bacterial Protein Extraction Reagenzien gewonnen werden

Lushchak, InTech, 2012 , 189

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E11G0369 Product Name Bovine Glutathione peroxidase ELISA kit Species Bovine Product Size 48 Tests / 96 Tests Concentration 5.0-100 ng/ml Sensitivity 1.0 ng/mL Principal Competitive ELISA Sample Volume 100 ul Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Time 90 minutes Platform Microplate Reader Conjugate HRP Detection Method Colorimetric Storage 2-8C Kit Components MATERIALS SPECIFICATION QUANTITY MICROTITER PLATE 96 wells stripwell ENZYME CONJUGATE 6.0 mL 1 vial STANDARD A (0.5mL) 0 ng/mL STANDARD B (0.5mL) 5.0 ng/mL STANDARD C (0.5mL) 10 ng/mL STANDARD D (0.5mL) 25 ng/mL STANDARD E (0.5mL) 50 ng/mL STANDARD F (0.5mL) 100 ng/mL SUBSTRATE A 6 mL SUBSTRATE B STOP SOLUTION WASH SOLUTION (100 x) 10 mL BALANCE SOLUTION 3 mL Principle of the Assay GSH Px ELISA kit uses an anti-GSH Px antibody and an GSH Px-HRP conjugate in a competitive enzyme immunoassay method