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Circulation 101(15):17671772 Gurrola-Diaz CM, Sanchez-Enriquez S, Oregon-Romero E, Garcia-Lopez PM, Garzon de la Mora P, Bastidas-Ramirez BE et al (2009) Establishment of a cut-point value of serum TNF-alpha levels in the metabolic syndrome

After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)