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The effects of a high-energy diet on hippocampal-dependent discrimination performance and blood-brain barrier integrity differ for diet-induced obese and diet-resistant rats
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Sections were washed in wash buffer and reacted with a mixture of highly cross-absorbed species-specific donkey anti-guinea pig IgG-Alexa 488 (1:300) and donkey anti-mouse IgG-Alexa 568 (1:600), diluted in 0.2% vol/vol Tween-20 in PBS for 1 h at 37 C
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