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After incubation, the supernatant was removed, and cells were fixed with 1% glutaraldehyde for 1 h before replacing the fixative with PBS

That three-receptor approach is what separates it from Tirzepatide (dual agonist) and Semaglutide (single agonist), and the clinical data reflects that gap clearly

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Reduced levels of NR2A and NR2B subunits of NMDA receptor and PSD-95 in the prefrontal cortex in major depression