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Reusing a single-dose pen risks contamination and dosing error Site rotation: Rotate injection sites each week
Cell culture Human umbilical vein endothelial cells (HUVECs), purchased from ScienCell (CA, USA), were maintained in endothelial growth medium (EGM, ScienCell) containing penicillin (10 U/mL), streptomycin (10 g/mL), and 5% heat-inactivated fetal bovine serum (ScienCell) as well as endothelial growth factors
Traceability: HPLC/MS documentation supports batch consistency across experiments
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