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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

Cells were washed twice with phosphate-buffered saline (PBS) and starved in DMEM containing 0.1% human serum albumin for 1 hour before stimulation with increasing concentrations of insulin or IGF1 for 30 minutes at 37C

Int J Nanomed 12:53875397