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Briefly, cells were treated with permeabilisation buffer for 5 minutes and then were centrifuged at 3000 g for 5 min to collect the cytosol fraction in the soluble fraction
Nature 300 , 276278 (1982)
The shRNA sequence against GLUD1 and siRNA sequences against PRMT7 were as follows: sh GLUD1 #1: 5- GCCATTGAGAAAGTCTTCAAA-3
Free Radical Biol Med 89:5461 Saleh MA, Antar SA, Hazem RM, El-Azab MF (2020) Pirfenidone and vitamin D ameliorate cardiac fibrosis induced by doxorubicin in Ehrlich ascites carcinoma bearing mice: Modulation of monocyte chemoattractant protein-1 and Jun N-terminal kinase-1 pathways
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