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Islets were then selected and transferred into wells of 96-well plates containing 0.2 mL of KRB with 20 mM glucose and various test compounds as indicated and incubated for an additional 60 min

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Mucosal extracts for quantification of GLP-1 Frozen lean or HFD obese rat gastric mucosal scrapings and human fundic and antral biopsies were homogenized (TissueLyser II, Retsch France) with an ice-cold buffer containing 1.5% HCl 1 M and 75% ethanol