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Samples each containing approximately 500 g of protein in SDC lysis buffer (100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [HEPES], 1% sodium deoxycholate [SDC], pH 8.1) were reduced and alkylated using 10 mM tris(2-carboxyethyl)phosphine (TCEP Sigma Aldrich) and 40 mM of chloroacetamide (CAA) at 95 C for 5 min

PekCura Labs is not a compounding pharmacy or chemical compounding facility as defined under 503A of the Federal Food, Drug, and Cosmetic Act

Tang LJ, Luo XJ, Tu H, Chen H, Xiong XM, Li NS, et al

By pairing two long-acting peptides, investigators can compare combined signaling with each component alone and assess whether the time course or magnitude of effects differs under controlled conditions