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Inject at the center of the triangle
You might notice weakness in foot dorsiflexion, the ability to pull your toes upward
On an experimental day, cells were washed with Hanks Balanced Salt Solution (HBSS, Gibco/ThermoFisher Scientific) and the respective receptors were labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corporation, Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/ThermoFisher Scientific) (2 h, 37 C, 5% CO 2 )
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