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A 5mg vial contains 5mg whether you add 1ml, 2ml, or 3ml
In addition to being administered as a drug to cause oxidative stress, MTX has also been used to induce cachexia, anorexia, intestinal mucositis and digestive absorption problem in experimental animal models (Reference Boukhettala, Leblond and Claeyssens34Reference Sinno, Coquerel and Boukhettala36)
For others, absorption may be limited due to digestive concerns, medications, or individual differences
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