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Experimental studies often analyze intracellular communication pathways regulating metabolic enzyme activity and biochemical signaling related to cellular energy processes
Hydrogen bonds were additionally analysed using the COOT, and the UCSF Chimera package, with relaxed distance and angle criteria (0.4 and 25-degree tolerance, respectively)
The protein content of the sample applied to the apical side (g/cm 2 ) was calculated as follows: protein content of digesta (BCA assay, g/mL)/ dilution factor [15]/volume on apical side (0.5 mL)/ Transwell filter area (1.1 cm 2 )
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