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Endogenous peroxidase activity was blocked with 0.3% HO, followed by permeabilization with PBST (1 PBS containing 0.1% Triton X-100)

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Glutathione steps in to scavenge these free radicals and prevent cellular injury

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Studies have documented MC receptor binding at all five subtypes, with particular focus on MC1R-mediated pigmentation responses and MC4R-mediated CNS pathway modulation in rodent models

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