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Bacteria were further incubated at 37 C for 4 h before being centrifuged at 5000 g for 10 min
Handling and Storage Recommendations Store unopened vials at 28 C, with 20 C recommended for long-term storage Protect from light and moisture After reconstitution, store solutions at 28 C and use within laboratory-defined timeframes Avoid repeated freeze-thaw cycles Follow standard laboratory safety, labeling, and handling procedures All handling and storage are carried out within laboratory research environments
The number of flinches was counted for a 1-min period at each minute interval from 1 min to 10 min (phase 1) and at 10-min intervals from 10 min to 60 min (phase 2) after formalin injection
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