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Peripheral blood mononuclear cells (PBMCs) from healthy donors, obtained from peripheral blood or buffy coats, were used as control

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After stirring for another 1 h at 25 C, P3 (300 mg) was introduced to the reaction mixture

The exact influence of circulating Th17 cells on depression remains unclear, but since Th17 cells can infiltrate the brain parenchyma without requiring VLA4 signals, it can be hypothesized that Th17 cells may reach the brain and synthesize IL-17 (68)