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The proportion of the dark nuclear staining cells was evaluated by counting the cells stained with TUNEL divided by the total number of cells

[ 1 ] Delta waves are low-frequency, high-amplitude brain oscillations that characterize slow-wave sleep, the deepest stage of NREM sleep associated with physical restoration, memory consolidation, and metabolic recovery

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In practice, stacking multiplies uncertainty: more compounds more chances of impurity/contamination more pathways affected more interaction risk more variables harder to know what helped (or harmed) If you take only one clinical principle from this article: the more experimental the stack, the more conservative your risk posture should be