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To isolate single-cell clones, antibiotic-selected polyclonal GGT1 KO cells were seeded at low density (50 cells per 15 cm dish) and grown for 2128 days
Cai et al., 2025)
In agreement with prior literature [35], this result suggests that GSH is not stable in plasma at 4 o C, and thus samples should not be stored at this temperature
Adding traditional fasting on top may amplify metabolic stress, raise hypoglycemia risk, or cause electrolyte imbalances unless a qualified provider is monitoring, which is why the post treats fasting as a supervised decision
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