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After blocking with 5% BSA in TBST, slides were stained with rat anti-BrdU antibody (which detects CldU but not IdU, OBT0030, 1:2000, AbDSerotec) and mouse anti-BrdU antibody (which detects IdU but not CldU, 7580, 1:1000, BD Biosciences) at 4 C for overnight

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