Pre-treatment of HGF with trisulfides and nitration Recombinant mouse HGF (carrier protein-free) was pre-treated with glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS) at mole ratios (HGF : trisulfide) of 1:0 (with solvent alone), 1:400, 1:1000, and 1:4000 at pH 7.4, 25C just prior to the peroxynitrite protocol (optimized at a molar ratio 1:2000 relative to HGF, pH 7.4, 37, for 30 min), which was previously demonstrated to induce maximal nitration of Y198 and Y250 located in the -chain under physiological conditions as described previously 32
A close analogue of GHK-Cu (differing by a single N-terminal methyl group), it is provided as a lyophilized powder with 99% HPLC purity, MS confirmation, and a third-party Janoshik COA
Research destination country laws before traveling internationally with peptides
Imai, H., Matsuoka, M., Kumagai, T., Sakamoto, T