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10.1038/jcbfm.2011.69 53 ClaassenJAHRThijssenD

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The PCR conditions involved an initial step at 95 C for 3 min, followed by 25 cycles of 95 C for 30 s, 55 C for 30 s, 72 C for 30 s, and a final extension of 72 C for 5 min